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Assessment of Genotoxicity of Levosimendan in Human Cultured Lymphocytes

[ Vol. 12 , Issue. 2 ]

Author(s):

Abeer M. Rababa'h*, Omar F. Khabour, Karem H. Alzoubi, Dua'a Al-momani and Mera Ababneh   Pages 160 - 165 ( 6 )

Abstract:


Background and Objective: Levosimendan is a positive inotropic and a vasodilator agent with pleotropic characteristics that include antioxidation, anti-inflammation and smooth muscle vasodilation.

Methods: In this study, the effects of levosimendan (0, 0.1, 1, 10, and 20 µg/ml) on oxidative DNA damage and sister-chromatid exchanges (SCEs) were evaluated in human cultured lymphocytes.

Results: The results showed that levosimendan increased the frequency of SCEs in all examined concentrations (P<0.01) except for 0.1 µg/ml. On the other hand, levosimendan did not induce oxidative DNA damage as measured by the 8-OHdG biomarker (P > 0.05). In addition, neither mitotic arrest nor proliferation index was affected by levosimendan at all examined doses (P > 0.05).

Conclusion: In conclusion, levosimendan might be associated with increases in sister-chromatid exchanges in cultured human lymphocytes. In vivo studies are required to confirm the present findings.

Keywords:

Levosimendan, mitotic index, 8-hydroxy-2-deoxy guanosine (8-OHdG), sister chromatid exchanges.

Affiliation:

Department of Clinical Pharmacy; College of Pharmacy, Jordan University of Science and Technology, Irbid, Department of Medical Laboratory Sciences, Faculty of Applied Medical Sciences, Jordan University of Science and Technology, Irbid, Department of Clinical Pharmacy; College of Pharmacy, Jordan University of Science and Technology, Irbid, Department of Medical Laboratory Sciences, Faculty of Applied Medical Sciences, Jordan University of Science and Technology, Irbid, Department of Clinical Pharmacy; College of Pharmacy, Jordan University of Science and Technology, Irbid

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